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Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography

All cell lines tested negative for mycoplasma contamination using a MycoAlert Mycoplasma Detection kit (Lonza, LT07-418)

The carotenoid may act as an antioxidant and lower inflammation

At each sampling point, cellular Fe 2+ was oxidised to Fe 3+ by addition of potassium dichromate (40 mM final concentration) to prevent further peroxide bond activation, followed by protein precipitation with acetonitrile (containing 150 ng/mL of diazepam as the internal standard) as previously described 28

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