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Administration Time Administer peptides at optimal times:- **PT-141:** 45-60 minutes before sexual activity- **Sermorelin:** At bedtime for optimal GH secretion- **GHK-Cu:** Apply morning and night to clean, dry skin or scalp- **NAD+:** Subcutaneous injection 1-3 times per week or oral daily Dosing/Settings Standard dosing protocol:- **PT-141:** 1.0-1.75 mg subcutaneous as needed, max 2x per week- **Sermorelin:** 200-500 mcg subcutaneous daily- **GHK-Cu:** Apply a thin layer to the treatment area twice daily- **NAD+:** 50-500 mg subcutaneous per dose, 1-3x per week Test Spot Requirement Recommended for first-time GHK-Cu users

doi: 10.1111/j.1574-6968.2001.tb10589.x