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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

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This is promising, but it is important to be accurate: this was not the same as proving that every topical GHK-Cu hair serum will regrow hair in real-world consumers

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