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Initially, the alterations in intracellular protein concentrations post-treatment were assessed, and the principal component analysis (PCA) outcomes demonstrated a notable divergence in protein expression between the D-CuP-treated group and the PBS-treated control group (Fig

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Firstly, we verified across a panel of normal and cancer cell lines with differing p53 status (Supplementary Table 1) that treatment with a lethal dose of APR-246 resulted in higher intracellular ROS (Fig

Together, these results indicate that decreased GSH/GSSG redox/antioxidant capacity and increased oxidative stress in the autism brain may have functional consequence in terms of a chronic inflammatory response, increased mitochondrial superoxide production, and oxidative protein and DNA damage