Cells were lysed in 100 L ice cold 8 M Urea/50 mM Tris HCl lysis buffer (Fisher Scientific) with 1:100 of Halt Cocktail Inhibitor, EDTA-free (100X) (Thermo Scientific)
Overuse or inappropriate administration may lead to nutrient imbalances
Temperature excursions above 8C accelerate this timeline even brief exposure to room temperature (25C) for 24 hours reduces stability to 14 days or less
The symptoms of a B-12 deficiency include numbness or tingling of the extremities, fatigue, weakness, anemia, swollen tongue, jaundice, difficulty walking, cognitive difficulties, memory loss and paranoia
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