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Mouse embryonic fibroblast cell line NIH/3T3 (#CRL-1658, ATCC), 293 T cells (#CRL-3216, ATCC) and normal liver cell NCTC1469 (#CCL-9.1, ATCC) were cultured in DMEM medium (#6123047, Gibco) with 10% FBS

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Causes of Leaky Gut Syndrome: Microbiota dysbiosis Small intestine bacterial overgrowth (SIBO) Gluten and Celiac Disease Food allergies Mast cell activation syndrome Fungal infections Toxicants Mold NSAIDs (Tylenol, Advil) Chronic alcohol consumption Strenuous exercise Nutritional deficiencies Aging Does SIBO Cause Intestinal Hyperpermeability

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