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The samples were vortexed vigorously for 30 seconds and held on ice

Animal experiment All procedures for animal experiments in this study were authorized by the Institutional Animal Care and Use Committee (IACUC) of Seoul National University (Approval Number: 220038-S1A0) and complied with the guidelines for the care and use of laboratory animals

From regional dysbiosis to inter-organ communication The previous chapter systematically reviewed the diverse microbial communities colonizing different anatomical sites in dairy cows and their functional characteristics (Tables 1 and 2), highlighting the close associations between these microbes and host metabolic homeostasis, immune regulation, and disease development

This assay was applied to both cultured cells and frozen tissue sections, with tissue sections counterstained with Nuclear Fast Red for better visualization