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PIS was solubilized in buffer containing 0.9%(v/v) Triton X-100, 5%(v/v) glycerol, 0.5 mM DTT, 10 M PMSF, 30 mM MgCl 2 , 1 mM MnSO 4 , and 20 mM Tris-HCl pH 8.5

Polyphenols appear to be effective chemopreventive drugs due to a number of protective actions they exhibit, including cell cycle signaling modification, activation of antioxidant enzymes, apoptosis, and cell cycle arrest

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As such, this amino acid molecule is more resistant to in vivo interactions compared to cysteine on its own

Briefly, spectra derived from unlabelled samples were processed through the deconvolution software AMDIS (Stein 1999), using the Fiehn library as database (Kind et al