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Dual luciferase assay Fragments of either CBS-3UTR-WT or CBS-3UTR-Mut (where m 6 A was changed to T) were cloned into pmirGLO vectors to generate CBS-3UTR-WT and CBS-3UTR-Mut plasmids (Table S5)

Studies demonstrate that Dihexa promotes dendritic spine formation, increases synaptic density, and enhances miniature excitatory postsynaptic currents (mEPSCs), indicating the formation of functional glutamatergic synapses

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Image analysis software was used to read the band intensity of the target proteins against the control sample after normalization by -actin ( 2.7 Histopathological analysis Hepatic injury can be detected by hematoxylin and eosin (H&E) staining of liver tissues ( The formalin-fixed liver specimens were inserted in liquified paraffin wax and cut into 5-m thick sections utilizing a Leica microtome (Leica RM 2155, England)