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The percentage of Ki67-positive cells in the hair bulb was quantified based on DAPI-stained nuclei

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Discrimination index The preference for and therefore recognition of the novel object, the novel arm in the Y maze, or the mouse over the empty cage were assessed by calculating the discrimination index (Ennaceur & Delacour, 1988): The discrimination indices of all genotype and treatment groups were compared individually with a theoretical value of 0, which would indicate no preference for the novel or familiar object, Y maze arm, or cage with mouse over empty cage using a one-sample t test

Our data confirmed the existence of the mentioned transcripts in hiPSCs-derived MVs, which indirectly supports the possibility of applying MVs for wound-healing

Dose Range: 2.5 mg/day to start, increasing toward 5 mg/day