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Endothelial NO/cGMP signaling To mimic the experimental conditions of the isometric tension studies with isolated aortas, cultured endothelial cells were incubated for 24 h with the extracts, followed by determination of L-arginine-to-L-citrulline conversion and accumulation of cGMP in response to activation of endothelial NO synthase by the Ca 2+ ionophore A23187