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Funding This work was supported by the grant RF-2013-02359621 from Ministero della Salute, Italy

Both vitamin B12 deficiency and folate deficiency are more common in older people, affecting around 1 in 10 people aged 75 or over and 1 in 20 people aged 65 to 74

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As detected by DCFH-DA staining, GCLC overexpression reestablished cellular ROS levels in cells expressing shACTL6A, phenotypes remained significant when treated with H 2 O 2 and was reversed by NAC (Fig