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Beyond the smoking traits, with all SNPs included, higher ND was genetically correlated with higher risks of alcohol dependence 30 , neuroticism 31 , psychiatric diseases (attention deficit hyperactivity disorder 32 , bipolar disorder 33 , major depressive disorder 34 and its symptoms 31 , posttraumatic stress disorder, and schizophrenia 35 ) and smoking-related consequences (lung cancer and its histological subtypes 36 and coronary artery disease 37 )

39 Immunofluorescence staining For immunofluorescence staining, colons from the various groups of mice were cut into 3 m sections via a freezing microtome

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