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By extension, evolutionary acquisition of structural disulfides in proteins can potentially occur via transition through a redox-active disulfide state

How Serial Dilution Works Reconstitute the peptide at a high concentration (e.g., 2 mg/mL) Take an aliquot and dilute 1:1 with bacteriostatic water 1 mg/mL Take an aliquot from the 1 mg/mL solution and dilute 1:1 0.5 mg/mL Continue as needed for the research protocol Important : Each dilution step must use sterile equipment and fresh bacteriostatic water

PMID 2422758

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Third, besides the analgesic role of cortistatin, we demonstrated that cortistatin administration avoided the progression of motor deficits after sciatic nerve injury and recovered the normal pain threshold, and that these beneficial effects were accompanied by an enhancement of axonal regeneration and a partial reduction in the muscle atrophy