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The left ureter was exposed via a left flank incision, ligated with 4-0 silk at two points, and cut between the two ligation points

They also assessed gut microbial composition by culturing fecal samples on selective media

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Ice-cold MeOH (600 L), CHCl 3 (200 L), and H 2 O (500 L) samples were vortexed and then centrifuged (10,000 g , 10 min, 4 C) to precipitate proteins