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We previously described purification, biochemical characterization and mass spectrometry analysis of each of TrxR1 variants to determine activity and verify incorporation of AcK at K141, K200, or K307 as well as Sec incorporation at the key active reside Sec551 (Wright et al., 2018)

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Level A Anchor Evidence: Unlike the SCFAsmodule, the Bile Acid/Tryptophan module (and broader comprehensive metabolic reprogramming) lacks Level A closed-loop anchors that converge the causal chain to specific bile acid or indole metabolite molecules

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Natural compounds are uniquely suited to target this downstream effector phase because many simultaneously attenuate inflammatory signaling and directly suppress endothelial CAM expression, thereby reducing leukocyte recruitment and preserving renal microcirculation