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Figure 4c shows the relative abundance of metabolites between PRKAG2 -mutant and isogenic control CMs, revealing several metabolic changes despite the overlapping profiles in the PCA

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Non-Responders: While most individuals benefit from creatine supplementation, a small percentage of people might not experience significant improvements in muscle strength or performance

The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)