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10.1007/s00406-006-1004-4 64

[21] The smoking age in Hawaii was 18 in 1988

The hCMEC/D3 cells (passage 2832) were seeded on collagen coated culture flasks (2.5-3 10 4 /cm 2 ) or glass slides (4 10 4 /cm 2 ) and maintained at 37C with 5% CO 2 exposure in EBM-2 basal medium (Lonza, Walkersville, MD, USA) supplemented with 5% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), chemically defined lipid concentrate (Life Technologies, Carlsbad, CA, USA), growth factors, antibiotic/antimycotic (1:1, Atlanta Biologicals, GA, USA and HEPES (10 mM)

These problems with the mens sperm could influence their fertility