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Cells were incubated in Maxpar PBS with the reactive dye for 1530 min at 37 C, washed, and additionally stained for T cell immune profiling

Fluorescent images were captured with the Zeiss LSM 510 Meta Inverted Confocal Microscope through the Vanderbilt University Cell Imaging Shared Resource

Stress has been linked to the development of most neuropsychiatric diseases, such as anxiety, depression, and insomnia, and can alter the structural and functional makeup of the brain [68, 69]

The gut microbiome and xenobiotics: identifying knowledge gaps

The human and mouse CYP51A1 promoters contain a CRE element in addition to the SRE element, suggesting two major regulatory pathways-sterol-dependent regulation and cAMP-dependent regulation