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A quantitative real-time polymerase chain reaction (PCR) was performed with PIKO REAL 96 instrument (Thermo, MA, USA) and the SYBGREEN PCR Mix Mix (Applied Biosystems, CA, USA) in accordance with the manufacturers protocol

Statistical analysis Students t -test and the chi-square test were used to compare the baseline characteristics of participants across groups

Credit: Hestia Tobacco LLC Though faced by overwhelming competition from big tobacco, craft cigarettes have carved out very individual niches for themselves

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