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For one-step RT-qPCR, the SuperScript III Platinum One-Step Quantitative RT-PCR System with ROX (Thermo Fisher) was used

Low Awareness: Many Indonesians are unaware of the long-term health risks associated with smoking, often believing myths like certain cigarette brands are healthier. The prevalence of smoking in Indonesia underscores the need for greater education and stricter government policies to curb this public health issue

"The tobacco companies are still spending a significant amount of their marketing budgets on high-risk products and, while a handful have increased their focus on youth access prevention, the impact of these policies is still unclear," said Dr

Packaging: Luxurious, elegant packaging adds to the premium feel and makes it a collectible item for enthusiasts

Sun or air curing involves leaving the tobacco to dry naturally which can take several months