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Because these groups are more likely to continue to smoke despite cessation efforts, it seems likely that more aggressive efforts may be needed to reach them and to change their behavior

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2 L of RNA was used in a reaction containing a final concentration of 1x Reaction Mix with ROX, 300 nM of forward primer, 300 nM of reverse primer, 100 nM of probe and 0.4 L of SuperScript III RT/Platinum Taq Mix

If you dont smoke, try to stay away from secondhand smoke