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Soluble His-tagged GpoA protein was purified using nickel affinity chromatography following manufacturers instructions (Ni-NTA agarose, Qiagen)

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B12 replacement produces the clearest results in people whose levels are low

Interactions with medications Vitamin B12 can potentially interact with certain medications and prevent their efficacy or cause side effects

In human studies, statistical differences between Control and HyperSperm groups were analyzed using Wilcoxon matched-pairs signed rank test for safety analysis, Fishers exact test for fertilization and embryo development, or a log-rank test (Mantel-Cox) for developmental times