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Nicotine, nornicotine, HPBA, NOX, Nic-Gluc, cotinine, COX, Cot-Gluc, 3HC, and 3HC glucuronide (3HC-Gluc) standards were purchased from Sigma-Aldrich

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Additionally, the aim was to investigate whether EC use is associated with increased risk of tobacco smoking and consumption of other substances, and what evidence exists regarding the use of ECs as a harm reduction strategy or for smoking cessation in adolescents

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