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The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

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found that the expression of metal ion transporters solute carrier family 39 member 14 (Slc39a14) and solute carrier family 39 member 8 (Slc39a8) is markedly upregulated during vascular calcification