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flexible ratios Cycle driver Whichever compound titrates more slowly Cagrilintide Loading 0.25 2.4 mg over ~16 weeks Frequency Once weekly Maintenance 2.4 mg Route SubQ Semaglutide Loading 0.25 2.4 mg over ~16 weeks Frequency Once weekly Maintenance 2.4 mg Route SubQ Weekly Schedule Example (Maintenance) Same day each week Cagrilintide 2.4 mg SubQ + Semaglutide 2.4 mg SubQ Use separate syringes for each compound

Quantification of H 2 S and total persulfide from mice heart exposed to Langendorff model of ischemia-reperfusion Snap frozen mouse heart obtained from Langendorff experiments was homogenised using Percellys tubes (approximately 10 mg tissue per 300 L of 50 mM HEPES (pH 7.4) buffer containing 10 mM IAM-TPP) two times for 30 s

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