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The above studies suggest that the hepatoprotective activity of salvianolic acid B against APAP is achieved by inhibiting cytochrome P450 enzymes and/or synergizing phase II metabolic enzymes

Briefly, INS-1E cells were maintained in RPMI 1640 medium (Gibco) supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 2 mM l-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 100 U/mL penicillin/streptomycin and 50 M 2-mercaptoethanol

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