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Prior work from our lab has demonstrated that even in the absence of caustic smoking-related stimuli, TCCSUP cell-derived EVs can promote the malignant transformation of immortalized human urothelial SV-HUC cells in a cargo protein-dependent manner 40

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As for the enzymatic process, nicotine is converted into cotinine, which is the main metabolite, ensuring that around 7080% of nicotines filtration is done through the liver

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