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Kim J, Kim H

In studies of human alveolar epithelial cells, treatment with nontoxic doses of CS exposure was found to induce mitochondrial hyperfusion and increase the expression of mitochondrial fusion-related proteins such as MFN1

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After deparaffinization, antigen retrieval and permeabilization, the samples were blocked and probed with the rabbit polyclonal antibodies against loricrin, involucrin (both Abcam, UK), filaggrin, KLK7 and TG1 (all three Invitrogen, USA) and appropriate anti-rabbit secondary antibody conjugated with Alexa Fluor 555 (Invitrogen, USA)