We separated the growth media from cells grown at a fixed temperature by flowing the liquid cultures through 0.45m-pore filters as previously described
2.2 Chemicals and reagents Puerariae Lobatae Radix (Gegen, #210501), Scutellariae Radix (Huangqin, #220501), Coptidis Rhizoma (Huanglian, #220301), and Glycyrrhizae Praeparata cum Melle Radix et Rhizoma (Zhigancao, #211101) were purchased from Shizhen Pharmaceutical (Guangdong)
Nature 492 , 285289
doi: 10.3389/fnins.2021.587197 Received 25 July 2020 Accepted 10 February 2021 Published 16 March 2021 Volume 15 - 2021 Edited by Anwen Shao, Zhejiang University, China Reviewed by Yi Yang, First Affiliated Hospital of Jilin University, China
In our discussion of the proposed MS-DRG reclassification changes, we refer to these claims data as the December 2022 update of the FY 2022 MedPAR file. Specifically, as discussed further in this section, we used the additional claims data available in the December 2022 update of the FY 2022 MedPAR file to assess the application of the NonCC subgroup criteria to existing MS-DRGs with a three-way severity level split, as well as to simulate restructuring of any proposed MS-DRGs, to assess the case counts and other criteria for determining whether a proposed new base MS-DRG would satisfy the criteria to create subgroups
