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Even though e-cigarette emissions generally contain fewer distinct chemicals than conventional cigarette smoke, they are by no means safe, as the inhaled particles are predominantly ultrafine in size and can deposit deeply in the lungs (37), as illustrated in Figure 1, where ultrafine particles travel through the airways and accumulate in the distal alveolar regions

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The 8-OHdG fraction was collected, depending on the relative elution position from the peak of the added marker, 8-OHG, and was automatically injected into the HPLC-2 column

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