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"This enabled us to investigate the effects on endothelial cells of substances in smoke/aerosol that are absorbed through the lungs and enter the bloodstream, and also any other circulating molecules that the body might produce in response to inhaling the smoke and/or aerosol, including proteins involved in the inflammatory response." The study recruited 120 adults between the ages of 21 and 50 who were free of cardiovascular disease

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Covariates measured at age of 1213 years included: 1) age