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As a general principle, the longer you can abstain before surgery, the better

RNAScope FISH (tracheal digest cytospin labeling) Tracheal cells prepared for 10X scRNA-seq were distributed on slides via centrifuge at 55.3 g , fixed for 15 min in 3.2% PFA/1.5% sucrose at room temperature, rinsed with 1 PBS and stored at 4 C

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