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The term "spliff" is sometimes used to distinguish a joint prepared with both cannabis and tobacco, [12] as is commonly done in European countries, where joints containing only cannabis are uncommon

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At 80% confluence, cells were detached with 0.05% trypsinEDTA (Gibco) and seeded on membrane supports (12 mm Transwell culture inserts, 0.4 m pore size, Costar) coated with 0.05 mg collagen from calf skin (Sigma-Aldrich) in ready-to-use AEGM supplemented with 1% penicillin/streptomycin