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In addition, reductions in systolic function [94], ejection fraction [95], increased systemic arterial resistance [96], as well as structural abnormalities [95, 96] have all been observed in rodent models exposed to aerosol with nicotine

The accuracy of the recorded data was validated in a pilot study, in which 5 vapers were asked to use the device for 30 puffs

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Total daily nicotine uptake Since humans differ widely with respect to the metabolism of nicotine to cotinine and subsequent elimination of cotinine, the use of cotinine as a marker of nicotine uptake has been questioned