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After this, 2 l of lysozyme (15 mg/ml), 180 l ATL buffer, and 20 l Proteinase K was added to the tube and incubated at 56C for 10 min after a brief vortexing

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Then, in his mid-sixties, he reached new audiences with his turn as George Costanzas irascible, voluble father on Seinfeld. A genetic basis for comedy is yet to be proved, but the couples two kids both joined the family business: their daughter, Amy, is a comedian, and their son, Ben, became one of the biggest movie stars of the past two decades, starring in films such as Theres Something About Mary, Meet the Parents, and Zoolander, which he directed

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