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Hourly images were then uploaded to Adobe Photoshop where they were cropped with lasso and highlighted with the paint bucket feature

Am J Respir Cell Mol Biol 53:159173 Hassan T, Carroll TP, Buckley PG, Cummins R, O'Neill SJ, McElvaney NG, Greene CM (2014) miR-199a-5p silencing regulates the unfolded protein response in chronic obstructive pulmonary disease and alpha1-antitrypsin deficiency

Exposure to second-hand smoke can trigger or worsen symptoms

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For controls, mice were exposed to a bluCig PLUS e-cigarette containing Gold Leaf tobacco (0% nicotine) or saline aerosol (27) (Afasci Inc, Burlingame, CA)