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For immunofluorescence, organoids or cultured cells were fixed, permeabilized with 0.1% Triton X-100, blocked with 3% bovine serum albumin and incubated with primary antibodies (for example, NRF2) overnight at 4 C

Disclaimer: Data and findings provided in the publications on this page reflect the content of this particular Surgeon Generals Report

Despite this decline, secondhand smoke is estimated to cause 41,000 deaths each year among adults in the United States, with 7,300 deaths attributed to lung cancer (195)