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Eluted peptides were ionized and sprayed into the mass spectrometer using an EasySpray Ion Source (Thermo Fisher Scientific) with a spray voltage of 1.6 kV and a capillary temperature of 275 C

Nitrotyrosine is considered a biomarker for oxidative stress as the result of the nitration of protein-bound and free tyrosine residues by reactive peroxy nitrate molecules formed when nitric oxide reacts with superoxide [8]

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