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PMID 27145839

SAM is actively synthesized, and all of the enzymes involved in the methylation process are present, including the methionine synthase pathway that regenerates methionine from Hcy

Centrifuge a suspension of the collagenase-dispersed cells at 50 g for 3 min

This recruitment initiates the assembly of the preinitiation complex, whose components include RNA polymerase II and general transcription factors (e.g., TFIID, TFIIB, and TFIIH), which collectively facilitate transcription initiation [146, 147]

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