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The criteria for liver injury were vacuolization of hepatocytes and pyknotic hepatocyte nuclei, activation of Kupffer cells and enlargement of sinusoids

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The limits of detection (LOD) and quantitation (LOQ) were established using drug-free matrix spiked with known concentrations of DOX (with IS), and the lowest concentration in the calibration curve was required to yield a signal-to-noise (S/N) ratio of 3 and 10, respectively

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