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The test product was then applied at varying concentrations (0.64, 3.2, 16, 80 M) to mouse macrophages for 24 h, and at concentrations of 3.2, 16, and 80 M to HFDPCs, followed by incubation for 24, 48, or 72 h

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The DNA receptor was prepared by removing water molecules, building in missing hydrogen atoms, and assigning atomic charges

Individual hair follicles were isolated and cultured in Williams Medium E (Sigma-Aldrich), supplemented with 2 mM L-glutamine (Sigma-Aldrich), 10 g/ml insulin (Sigma-Aldrich), 100 ng/ml hydrocortisone (Sigma-Aldrich), 0.1% fungizone (Gibco), 1% antibiotic-antimycotic (Gibco), and 1% Penicillin-Streptavidin (Gibco) under standard conditions (37 C, 5% CO2)

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