Representative tissue specimens (i.e., the brain, liver, kidney, pancreas, stomach, small and large intestine, lungs, and heart taken at the end of the experiment, fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h) were embedded in paraffin, sectioned at 4 m, and stained with hemalaun and eosin (H&E)
It is uncommon for the new fibrotic material to end up exactly where the original fibrosis occurs
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Even though the pH was not measured or reported in van Tonders paper, a deionized water solution of niclosamide has a pH of 6.5, (as measured in the present study) and the solubility obtained at pH 6.89 of 3.8uM 1uM, is in good agreement
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