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The -lactamase AmpC from Enterobacter cloacae WS1293 (UniProt ID: C0M550) was produced with and without a C-terminal Azo-tag in NEBExpress(lowRF1) using a 50 mL LB/Amp culture (see above)

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This approach maintains physiologic regulation and reduces the risks associated with direct HGH replacement therapy

Cells with APEX2 or HRP were enzymatically reacted with 2.5 mM DAB (Sigma-Aldrich, D8001-10G) with 4 mM H 2 O 2 (from 30% stock) in 0.1 M sodium cacodylate buffer at pH 7.4 for 320 min until the desired brown intensity color of the precipitate was visible

Genetic and morphologic diversity of Echinochloa crus-galli populations from different origins