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[DOI] [PMC free article] [PubMed] [Google Scholar] 88.Pedata F., Pugliese A.M., Coppi E., Dettori I., Maraula G., Cellai L., Melani A

Recent limits imposed on SDPs through the OBBBA will drastically reduce funding from this critical hospital payment mechanism and compound the impact of DSH cuts

c , The PDK1-silenced HCT116 cells stably expressing PDK1 WT or K163R were serum-starved and treated with insulin for 0 or 30 min, followed by IF with anti-Akt (red), anti-SETDB1 (green) and DAPI (blue)

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If required, cells were transfected with plasmid DNA using jetPRIME according to the recommended protocol of the supplier, one to two days before imaging