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2007, Munari and Gondzio 2013, Gondzio et al

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Poor absorption can also contribute to deficiency and may occur with digestive conditions such as inflammatory bowel disease, irritable bowel syndrome or coeliac disease

They will also set an upper limit on the total weekly dose (for example, some experts recommend not exceeding ~300 mg per week in subcutaneous dosing without further evaluation) to minimize any risk of adverse effects

Signal development was performed using DAB substrate (Beyotime, China), and sections were counterstained with hematoxylin (Solarbio, China)