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In vitro cytotoxicity assay Cells (HFD) in the logarithmic growth phase were seeded into 96-well cell culture plates (6000 cells/well) and incubated overnight at 37 C and 5 % CO 2

Cheedella NK, Suzuki A, Xiao L, Hofstetter WL , Maru DM, Taketa T, Sudo K, Blum MA, Lin SH, Welch J, Lee JH, Bhutani MS, Rice DC, Vaporciyan AA, Swisher SG, Ajani JA