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Neutrophils were isolated from mouse bone marrow of Nox2 wt and Nox2 / mice , using the MojoSort isolation kit, as described above, and (1 10 5 ) cells per gentotype, in triplicate, were pre-incubated in RPMI media with either NAC (5 mM), gp91-ds-tat (10 M) or DPI (10 M) for 30 min at 37 degrees

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Administered at doses of 5002000 mg/kg/day for 20 days, the treatment provided strong evidence of its neuroprotective efficacy (Sohn et al., 2021)