Representative confocal images of paxillin-labeled FAs in U-2 OS cells treated with indicated siRNAs followed by 48 h treatment with PBS or 25 mM 2-deoxy-D-glucose (2-DG)
doi: 10.1155/2016/1590952
The membranes were subsequently incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000, Proteintech, SA00001-2, Chicago, USA) at room temperature for 1 h, and the signals were visualised using an enhanced chemiluminescence detection reagent (Sigma-Aldrich, USA)
Avoid combining it directly with vitamin C or highly acidic products, which may destabilize the peptide
Modification strategies such as optimizing cell culture conditions, genetically modifying source cells, or refining Exos isolation techniques can increase the yield of EVs [237, 238]