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Flow cytometry analysis For surface staining, the cells were incubated with antibodies, including anti-CD3-AF700, anti-CD4-FITC, anti-CD45-BV510, anti-CD8-Percp-cy5.5, anti-GPR15-APC, and anti-CD25-BV650 (all from eBioscience), for 30 min at 4 C in the dark

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Subgroup findings Only four single-sex-based animal studies 29,49,51,52 were found analyzing oxidative stress in the lung, of which three studies were conducted in male mice 29,49,51 and one study in female mice 52

And just to seal the deal, they tossed in quirky covers of stuff like Black Flag s Wasted and Sonic Youth s I Love Her All the Time

Pods, mods, tanks, coils, I experimented with them all